phospho-pp2a (p-pp2a) antibody  (Cell Signaling Technology Inc)

Journal: Communications Biology

Article Title: PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation

doi: 10.1038/s42003-021-02175-1

Figure Lengend Snippet: Sections of E18.5 distal femur from Lepr-cre; Ppp2r1a fl/fl mice were subjected to immunostaining. a – d Immunohistochemistry (IHC) and immunofluorescence reveal that LepR + MSCs express Ppp2r1a and unphosphorylated (Y307) PP2AC in POC. a LepR IHC. b Ppp2r1a IHC. c Double immunofluorescence of LepR and Ppp2r1a. d Unphosphorylated (Y307) PP2AC IHC. e Illustration of conditional knockout of Ppp2r1a in LepR + MSCs. IHC reveals successful deletion of Ppp2r1a in LepR + MSCs in POC. IHC reveals that deletion of Ppp2r1a in LepR + MSCs leads to decreased expression of Ki67 ( f ) and hypertrophic markers, such as Runx2, Osterix, collagen X, MMP13 and alkaline phosphatase ( g ), and increased expression of Perilipin ( h ) in POC at E18.5. The rectangle box shows magnified picture. HZ = Hypertrophic zone; POC = Primary ossification center; ColX =collagen X; ALP = alkaline phosphatase. Scale bar, 100 μm.

Article Snippet: Sections were then immersed in PBS with 10% BSA for 30 min and then stained overnight at 4 °C with anti-LepR, anti-Ppp2r1a (Genetex GTX-102206, 1:100), anti-phospho Tyr307 PP2AC (Santa Cruz sc-271903, 1:50), anti-Ki67, anti-Osterix, anti-Runx2, anti-collagen X, anti-MMP13, anti-alkaline phosphatase, anti-Perilipin (Millipore ABS526, 1:100) and anti-human phospho Ser451 Runx2 (corresponding to Ser472 in the murine Runx2, Bioss bs-5685, 1:50).

Techniques: Immunostaining, Immunohistochemistry, Immunofluorescence, Knock-Out, Expressing

Journal: Communications Biology

Article Title: PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation

doi: 10.1038/s42003-021-02175-1

Figure Lengend Snippet: Sections of P15 distal femur from Lepr-cre; Ppp2r1a fl/fl mice were subjected to immunostaining. a–d Immunohistochemistry (IHC) and immunofluorescence reveal LepR + MSCs express Ppp2r1a and unphosphorylated (Y307) PP2AC in SOC. (a) LepR IHC. b Ppp2r1a IHC. c Double immunofluorescence of LepR and Ppp2r1a. d Unphosphorylated (Y307) PP2AC IHC. e IHC reveals successful deletion of Ppp2r1a in LepR + MSCs in SOC. IHC reveals that deletion of Ppp2r1a in LepR + MSCs leads to decreased expression of Ki67 ( f ) and hypertrophic markers, such as Runx2, Osterix, collagen X, MMP13 and alkaline phosphatase ( g ), and increased expression of Perilipin ( h ) in SOC at P15. HZ = Hypertrophic zone; POC = Primary ossification center; ColX =collagen X; ALP = alkaline phosphatase. Scale bar, 100 μm.

Article Snippet: Sections were then immersed in PBS with 10% BSA for 30 min and then stained overnight at 4 °C with anti-LepR, anti-Ppp2r1a (Genetex GTX-102206, 1:100), anti-phospho Tyr307 PP2AC (Santa Cruz sc-271903, 1:50), anti-Ki67, anti-Osterix, anti-Runx2, anti-collagen X, anti-MMP13, anti-alkaline phosphatase, anti-Perilipin (Millipore ABS526, 1:100) and anti-human phospho Ser451 Runx2 (corresponding to Ser472 in the murine Runx2, Bioss bs-5685, 1:50).

Techniques: Immunostaining, Immunohistochemistry, Immunofluorescence, Expressing